Hematological FISH probes offered

Interphase fluorescence in situ hybridization (IFISH) tests are available that can detect aberrations not apparent by G-banding analysis of chromosomes. Both metaphase and interphase cells can be examined by IFISH. IFISH tests cannot replace G-banded chromosome analysis, which detects abnormalities not targeted by the IFISH probes. Thus, the IFISH tests are best used as a complement to G-banded chromosome analysis and can be done using the same sample you send for karyotyping (1ml heparinized marrow). IFISH request form.

We offer the following probes for FISH testing of Hematologic disorders:

FISH probe panels
 
For disease:Chromosome anomalies detectedLoci or Genes
AML (M2, M3, M4)t(8;21), t(15;17), inv(16)ETO/RUNX1, PML/RARA, CBFB
CLL (or SLL)del(6q), del(11q), trisomy 12q, del(13q), del(17p)MYB, ATM, CEP12, D13S319, TP53
MDS (or CMML)del(5), -7 or del(7), +8, del(20)EGR1, D7S486/CEP7, CEP8, D20S108
MPDdel(5), -7 or del(7), +8, del(13q), del(20)EGR1, D7S486/CEP7, CEP8, D13S319/LAMP1, D20S108
Multiple Myelomat(4;14), t(11;14), t(14;16), del(13q), del(17p), +5, +9, +15IGH/FGRF3, IGH/CCND-XT, IGH/MAF, TP53/CEP17, D13S319, LAMP1, D5S23/D5S721, CEP9, CEP 15

For particular loci of interest:

Individual FISH probes
Chromosome anomalies detectedLoci or Gene
rea(2p23)ALK
rea(3q27)BCL6
rea(4q12)FIP1L1, CHIC2, PDGFRA
rea(8q24)C-MYC
t(8;14)MYC/IGH
t(8;21)ETO/RUNX1
rea(9p21)P16/CEP9
t(9;22) and deletion of the derivative chromosome 9 from the t(9;22), and ASS probe when necessary to r/o del(9)(q24)BCR/ABL* (Do not order if PCR test is negative)
rea(11q23)MLL
t(11;14)CCND1/IGH or CCND1-XT/IGH
t(11;18) OR t(14;18)API2/MALT1 OR IGH/MALT1
rea(12p13)TEL/ETV6
t(12;21)LSI TEL/AML1
rea(14q32)TCL1 OR IGH (Please specify)
t(14;18)IGH/BCL2
t(15;17)PML/RARA
inv(16)CBFB
rea(18q11)SYT
rea(22q12)EWSR1