Fresh Specimens

General Recommendations for the Handling of Fresh Specimens

Specimen Required: From resected specimen, sample a piece of the tumor (non-necrotic, trim away normal tissue). (A corresponding sample should be submitted for histologic diagnosis and designated as such in the gross dictation). Place sample in minimum essential media (MEM) plus 10% fetal calf serum and 10% DMSO. (If you are using your own transport media, which does not contain DMSO, do not freeze.)

Handling: It is important not to have the sample at room temperature. Exposure to room temperature can result in degradation, which can lead to false-positive aneuploid cell populations in the sample.

labeling: The specimen label should include the patient name, identification number, and specimen source.

Shipping: Ship the same day on wet ice.
Ship overnight on dry ice.
A Phone call to notify laboratory of shipment is appreciated. Please do not ship for weekend or holiday delivery.

Pathology Service Request Form Required:
Flow Cytometry DNA Analysis Report: Report contains analysis results including histogram, interpretation and supporting literature references.

Process Time: 4 days from receipt.

Fee:
Contact Person: Julie Moody
(206)790-3933
e-mail: fjulie@u.washington.edu
Flow Cytometry Laboratory:
Location: University of Washington Medical Center
Room NW-110

Contact for technical assistance:

(206) 598-6415
Contact for interpretation assistance: Peter Rabinovitch, M.D., Ph.D., Director
(206) 685-3761
e-mail: petersr@u.washington.edu