Protocols
Protocols for common experiments can be found in the links below.Proliferative Survival
Cell Cycle Analysis (dead) - DAPI
Cell Cycle Analysis (live) - Hoechst 33342
Apoptosis - Hoechst 33342-Syto 11-PI-CEN
Apoptosis - Hoechst 33342-CmXRos-MTG
Apoptosis - There are numerous commercially available kits and antibodies for measuring apoptotic parameters by FLOW cytometry, including the binding of Annexin V-FITC and exclusion of PI by early apoptotic cells.
If the procedure you are looking for does not have a link, you can find more procedures in our online lab manual.
Dyes we have that you can use for your experiments:
- DAPI Stock - 10mg/ml - with and without NP40 and DMSO
- Hoechst 33342 - 10mg/ml
- Propidium Iodide (PI) - 10mg/ml
- CMXRosamine
- Syto 11
- MitoTracker Green
- Ethidium Bromide (EB) - 10mg/ml
- Bromodeoxyuridine (BrdU)
- Chicken Erythrocyte Nuclei Singlets (CENs)
If the dye you need is not listed here, we may still have it. Please come to the lab and look in our Chemical Log for the dye that you need. The log book shows the location and amount of the dyes that we currently have in the lab.