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Procedures
- HUMAN CELLS MUST BE FIXED -
BRING:- Your samples resuspended at a concentration of at least 500,000/ml up to 25 million/ml - we can accomodate lower concentrations if needed, but please speak to us beforehand and let us know what concentration you plan to bring.
- Your samples should be in 5 ml polystyrene round-bottom tubes (Falcon 352052) - we have these tubes in the lab, so you can transfer them once you get here. Tubes must be labeled.
- A clearly written list of what is in each tube (either on paper or on the tube); positive, negative, sample #, etc. We will save your data according to what is written either on the tubes or on your list.
- Negative control
- Positive control
- Tubes or plates to collect sorted cells
Practices
What we can do for you:- Two-way sorting - 5ml polystyrene tubes, microcentrifuge tubes, 15ml tubes.
- Sterile sorting for tissue culture, upon request.
- Post-processing analysis - cell cycle or multi-color analysis.
- Samples can be cooled or heated in the sample chamber if they are temperature sensitive.
- Time can be monitored and results plotted on a time course for all experiments.
Data Access
If you would like to retrieve your raw data from our fileserver, you may obtain the user name
and password from us and access remotely through the following link:
Remote Data Access