Viable Hoechst 33342 Stain
- Cells should be maintained in the media that is normal for their growth.
- Add 10 µl of 1 mM Hoechst 33342/ml of media. Incubate for 30 minutes at 37°C. Optimal incubation time will vary with cell type.
- Cells must be maintained in Hoechst, DO NOT wash out the stain.
- Put the samples on ice after incubation in order to slow dye efflux.
- Run the samples on the flow cytometer as soon as possible (within minutes) of staining.
Hoechst 33342 (viable DNA stain) 1mM
For a 200 ml solution combine:
- 0.112 g Hoechst 33342*
- 200 ml of dH20
- 2 ml 95% ethanol
- Mix thoroughly. Cover the bottle with aluminum foil to protect from the light. Store at 4°C.
Bisbenzimide H 33342 Fluorochrome, Trihydrochloride.